Professionelle Corona Test Kits von AlphaMetrix

CytoBrite® Family

Precision heating instruments for FISH/ISH protocols

CytoBrite Slide Incubation System[more]
CytoBrite Duo
CytoBrite Duo Slide Incubation System[more]
CytoBrite Slide Oven
CytoBrite Slide Oven[more]
CytoBrite Workflow
1. Add probe and coverslip[more]

C. Dana Bangs - Cytogenetics Supervisor
Stanford Hospital and Clinics - Palo Alto, CA, USA

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(0.09 MB)
I have long been frustrated by dependence on individual hybridization instruments with linked denaturation and hybridization functionality. ?The linear addition of new instruments to meet increased test volume is limited by cost and available bench space. ?Suggesting to SciGene that the solution was to de-couple denaturation and hybridization with a high-capacity, high-precision incubation oven, mated to their CytoBrite hybridization instrument, quickly lead to the development of the CytoBrite Slide Oven. The CytoBrite Slide Oven incubates up to 60 slides in 6-slide hybridization trays transferred directly from the CytoBrite instrument following denaturation. Slides can also be transferred from HyBrite and ThermoBrite instruments. This frees the denaturation instruments for additional runs. The trays fit mated heat blocks on five pull-out shelves, facilitating rapid transfer of multiple slides with negligible temperature disruption. Precision (temperature regulation to ? 0.2?C) is excellent, and the use of CytoBond Coverslip Sealant eliminates the need to humidify the oven. The CytoBrite oven has successfully hybridized the full range of FISH testing, including standard cytogenetic interphase and metaphase preparations, FFPE specimens (breast, lung, lymphoma, sarcoma, etc.), and fixed intact cell Thin-Prep slides for bladder cancer screening. Probes from the major vendors and representing all FISH strategies (fusion, breakapart, deletion, amplification) have been successfully hybridized. Results have been excellent.

Dr. Mary Lowery Nordberg - Director of Molecular Pathology
Delta MDx - Shreveport, LA, USA

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(0.1 MB)
We were fortunate to participate in SciGene's Technology Access program for the CytoBrite Slide Incubation System when we were able to compare its performance to both the Hybrite and Thermobrite instruments. We were very pleased to find that the CytoBrite System eliminated a nagging problem we were having with ?spatter? with some probes and samples. My lab reported these results at the 2014 annual meeting of the Association of Genetic Technologies (AGT) in Louisville, KY.? We love the CytoBrite System and now use it on the full range of sample types and probes used in our laboratory including cell lines, bone marrow and paraffin-embedded samples with FISH probes from Abbott, Agilent, Cymogen and Cytocell. In addition to providing consistently better results, we love that we don?t have to fill the CytoBrite System with water absorbent pads since it uses CytoBond Sealant in place of rubber cement; eliminating humidification. Our technicians really, really like the CytoBrite 6-slide carrier which makes it easy to transfer slides in batches rather than one at a time. Another unique feature is the instrument?s front USB port which allows us to easily download a time and temperature datalog for each run. The CytoBrite System has become our GO-TO instrument for processing all paraffin-embedded samples since it is very controlled and provides the tightest temperature regulation of all our instruments. We place our most sensitive samples on the CytoBrite System and use our other instruments for outliers and samples less susceptible to temperature variation. As our samples increase we will likely purchase a companion CytoBrite Slide Oven to transfer slide trays from the CytoBrite System for the overnight probe denaturation step.


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